@article{Profil Kromatografi dan Uji Kemurnian Isolat Metabolit Sekunder Kulit Batang Lannea coromandelica (Kayu Jawa)_2026, volume={5}, url={https://ejournal.stikeskesosi.ac.id/index.php/Medlab/article/view/579}, DOI={10.57213/medlab.v5i2.579}, abstractNote={
Indonesia has abundant biological resources with potential for development as traditional medicine. One plant used by the people of South Sulawesi is Kayu Jawa (Lannea coromandelica), which is empirically used to treat burns, and as an analgesic, anti-ulcer, and antibacterial agent. This study aimed to perform the separation and isolation of secondary metabolite compounds from Kayu Jawa bark to determine the chemical profile and purity level of the isolates obtained. The thick extract was fractionated using the liquid-liquid fractionation (SCC) method, followed by analytical TLC, column chromatography, preparative TLC (PTLC), purity testing using two-dimensional TLC (2D-TLC), and determination of total flavonoid content using UV-Vis spectrophotometry. The fractionation results showed a yield of 16% for the n-hexane fraction and 4% for the ethyl acetate fraction. Column chromatography successfully separated the components based on their polarity, and the 2D-TLC results showed a single, compact spot under UV 254 nm and UV 366 nm illumination. The stem bark of Kayu Jawa (Lannea coromandelica) proved to be a potential source of secondary metabolites (alkaloids, tannins, flavonoids, triterpenoids), 2D-TLC testing confirmed that the isolate obtained had a good level of purity, and the total flavonoid content obtained was 17.648 mg QE/g or 1764.8%.
}, number={2}, journal={Jurnal Medical Laboratory}, year={2026}, month={Jul.}, pages={91–105} }